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It may be useful to incorporate the routine use of molecular protocols such as 16S rRNA PCR and sequencing as an initial screen for microbial pathogens in clinical samples, this would allow more selective and efficient use of traditional culturing for confirmation, the cost of the molecular methods being offset by using less traditional culture associated consumables.
A key challenge is to establish more widespread use of molecular protocols in developing world settings.
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Traditional bacterial culture protocols had failed to isolate the organism in a blood sample when the patient first presented 6.5 years previously; hence the discovery was made as a direct consequence of using molecular protocols.
The availability of free internet based molecular bioinformatic tools such as search engines and databases for molecular analysis represents a great resource for laboratories within developing countries, using or planning to use molecular protocols in their work.
We developed a simple protocol, which enables the use of molecular barcodes in high multiplex PCR with hundreds of amplicons.
List of loci and molecular protocols are described elsewhere [ 43].
Molecular methods were performed with the use of standard protocols (Ausubel et al. 1994).
Identical molecular protocols were used for analysis of questing nymphs.
Percentage of use of such protocols.
Percentage of use of such protocols 4.
Such patients can be included at an early stage in the right treatment protocols, much faster than by use of current molecular techniques.
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