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One such alternative diagnostic approach is the use of microarray platforms to detect RP mutations.
The use of microarray platforms has enabled a wider exploration of the potential association between gene expression patterns.
In fact, this is what we have learned from the use of microarray platforms: high throughput assay vendors are mainly focused on assay/hardware development.
Further functional investigation is required for such dynamically expressed NATs, and the use of microarray platforms targeting both strands of the gene locus will help to narrow down the proper candidates for further functional analyses.
The results of the MAQC project provide strong support for inter-platform consistency and reproducibility and support the use of microarray platforms for the quantitative characterization of gene expression.
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More recently, the Microarray Quality Control project (MAQC) compared gene expression measurements of two RNA samples using a number of microarray platforms, as well as alternative technologies, and demonstrated intraplatform consistency and interplatform concordance in terms of genes differentially expressed [ 20].
We checked the hybridization signal profiles of all recurrent CNV regions in the parents and assessed all previous work in the parents for CNVs which were not detected in our data but were detected in previously published work that used a range of microarray platforms and probe densities [ 21- 23].
We review the evolution of microarray platforms used for such studies in order to meet the criteria of complex tissue engineering biological environments.
Merging did not deteriorate performance on average despite (a) The diversity of microarray platforms used.
For instance, the diversity of microarray platforms used for gene expression analysis and the variability in microarray data emphasise the need for quality control.
Since alternative splicing has been largely ignored throughout the probe design of traditional expression microarrays, these findings have motivated the development of a new generation of microarray platforms, which use probes targeting individual exons to interrogate pre-mRNA splicing at the genomic scale [10] [14].
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