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A major advance was made in 2007 when Shinya Yamanaka found a way of avoiding all use of human oocytes.
Many Koreans are worried about the use of human oocytes for human embryonic stem [ES] cell research.
iPS technology makes reprogramming much easier [2,3] in comparison to early reprogramming methods such as somatic cell nuclear transfer (SCNT) [4,5], iPS technology also circumvents the ethical problems arising from the use of human oocytes.
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This medium composition was based on the formulation used for the IVM of human oocytes (Wynn et al., 1998).
Analysis of human oocytes with the use of NanoString and immunoblotting.
If ES cells could be a source of human oocytes, scientists might be able to use them for nuclear transfer experiments rather than using eggs from human donors, which are in short supply.
The only ethically acceptable manner to obtain large enough numbers of human oocytes for this type of a study was to use the GV and MI oocytes which cannot be injected with sperm, and mature them in vitro.
To evaluate a vitrification solution using a mixture of two cryprotectant agents, dimethyl sulfoxide and ethylyne glycol plus sucrose, on the survival of human oocytes.
"Mitochondrial DNA content affects the fertilizability of human oocytes".
To determine if long-term cryopreservation of human oocytes affects oocyte developmental competence, blastocyst euploidy, or live-birth rates.
To study the effect of FSH on the aneuploidy risk of human oocytes matured in vitro.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com