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The use of high buffer concentration was necessary to maintain a constant pH throughout the experiment.
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These include the use of high-salt buffers that are not compatible with electroporation conditions and may lead to arcing; the presence of contaminating (unreacted) dye in stock protein samples; and the presence of non-internalized fluorescent molecules that persist in the cell suspension even after thorough cell washing.
Therefore, the controlled-release discs allow a better control of the pH, than merely using high buffer concentrations.
Maintaining junction quality with ultra-thin window layers is facilitated by use of a high resistance oxide buffer layer, such as SnO2, In2O3 or Ga2O3, between the heteropartner and the transparent conductive oxide.
The most commonly used indicator for WSNs is high buffer occupancy.
The use of alkaline buffers and high temperatures are favorable for biomineralization.
The use of hot buffer extraction was efficient to obtain a high fucoidan yield with maintaining high sulfate contents.
Since GAD is active only at acidic conditions even though pH increases as the reaction proceeds, the conventional practice of using this enzyme involved the use of relatively high concentration of buffers, which might complicate the downstream purification steps.
For example, the frequently used TVA/TIC ratio can be biased due to the high buffer capacity of the ammonium/ammonia system.
In conditions favorable to protein adsorption, the use of anionic buffer leads to the adsorption of an apparent monolayer of proteins, while in zwitterionic buffer, proteins adsorb in a significantly higher amount.
It discusses the use of OpenCL buffer and image objects.
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