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The use of genome engineering in crop improvement has already revolutionised modern agriculture in less than thirty years.
The use of genome engineering techniques (Spitz et al. 2005; Diaz-Castillo et al. 2012) to perturb the interactions between HCNE peaks and their putative targets, coupled with expression assays (Woolfe et al. 2005), will help to more precisely dissect the role of HCNE peaks in impacting gene function within the context of chromosome repatterning.
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The goal was to initiate an informed discussion of the uses of genome engineering technology, and to identify those areas where action is essential to prepare for future developments.
We obtained the D15 and D19 alleles from experiments of genome engineering using ZFNs.
Recently, new technologies that use customized nucleases to facilitate targeted modification of genomic DNA have energized the field of genome engineering (Gaj et al. 2013).
Our work will be useful for the emerging field of genome engineering.
Our data will be useful for the emerging field of genome engineering.
The data obtained will be useful for the emerging field of genome engineering.
FokI is the same catalytic domain used in the ZFN and TALEN systems of genome engineering.
Open image in new window Figure 1 Applications of genome engineering in disease modeling.
Engineering zinc finger protein motifs for specific DNA targets in genomes is critical in the field of genome engineering.
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