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A further limitation of this work was the use of frozen mouse embryos.
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Briefly, total RNA was extracted from a small piece of frozen mouse liver using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions.
We used this procedure to amplify the genomes of 37 single cells from sections of frozen mouse tumor and normal lung tissues, which were micro-dissected and catapulted onto micro-tube caps.
Immunostaining of frozen mouse tumour sections to quantify blood vessel density was done using anti-PECAM antibody (BD Biosciences, San Jose, CA, USA) as described previously (Reynolds et al, 2002).
She recognised the advantages to biomedical science of cryopreservation of mouse mutants and strains; and the archive of frozen mouse embryos at Harwell, which provides such an important repository for biomedical science worldwide, is testament to her foresight.
The use of frozen embryos seemed to benefit mothers too.
In another, she defended the use of frozen peas.
RNA was isolated using TRIzol reagent from the frozen mouse tissue according to manufacturer's instructions.
Mice kidneys: Proteins were extracted from frozen mouse kidneys using RIPA [43] For microscopy, kidneys were fixed in 4% formaldehyde.
Make use of the mouse.
As a proof of principle we applied TACh to frozen mouse liver tissue.
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