Sentence examples for use of flp from inspiring English sources

Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the protein [6].

Use of FLP recombinase leaves a short FRT sequence as a scar.

Although not yet widely adopted in the liver, use of Flp- FRT may well expand in coming years.

Previously, we developed a selection marker gene (SMG -free SMG -freeific integration approach based on the use of FLP- FRT for gene integration followed by the usite-specificx for SMG-deletion [ 24].

Nevertheless, the advantages of FLP- FRT system in the i-PITT are, i) it allows removal of extra sequences for generation of the "clean" inserted allele (TIΔex allele) and ii) combinatorial use of FLP- FRT and Cre- loxP systems would result in increased targeted insertion frequency of DOI, as described below.

Making use of two FLP lines, T155-Gal4, UAS-FLP (hereferredreferred to as T155-FLP) and Hsp70-FLP, we are able to generate Smr mutant clones in various tissues including the wings, legs, and ovaries (the activity of T155 in the imaginal discs and ovary are shown in supplementary material Fig. S1A C).

The system makes use of either FLP or a low-activity FLP enzyme, termed FLPL.

In this paper I describe the use of the Flp site-specific recombinase for cloning PCR-amplified fragments.

The system developed by Datsenko and Wanner used expression of Flp recombinase which acts on corresponding Flp recombinase target (FRT) sites to excise the resistance marker from the genome leaving a 81 to 85 bp "scar" sequence [1].

In the current study we provide the first data supportive of the use of 293 Flp-In based stable mammalian system for influenza hemagglutinin expression.

HA (haemagglutinin) and Flag-epitope tagging of chromosomally encoded proteins was done as previously described [ 21] and the associated antibiotic cassettes were eliminated, when indicated, by the use of the FLP-expressing plasmid pCP20 [ 22].