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The oral consent of the patient was obtained for the use of fat sample for research.
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Another study using abdominal fat samples taken at 7 wk from a different population of divergently selected fat and lean chickens reported the differentially expression of 230 adipose genes [153 were up-regulated in the fat chickens, while 77 were up-regulated in the lean birds] [ 89].
The primary disadvantage of using RNA from milk fat samples appears to be its vulnerability to degradation, although in our study, this was only observed in human samples.
In a first attempt to test this hypothesis, we tested whether a fat sample which is used for an autologous fat graft can modify the growth of human osteosarcoma induced in immunodeficient mice.
Human fat sample was obtained from one female patient in the course of an aesthetic abdominal liposuction with her informed consent for the use for anonymized unlinked research.
We then demonstrate the effectiveness of the proposed method by the use of fresh onion samples and fat tissues excised from mice.
The origin of fat cells (fat samples from subcutaneous or visceral fat depots) could explain these discrepancies between studies.
Contributing factors include healthy diet and use of "healthy" fats in place of saturated fats.
Instead of focusing on calories or grams of fats, we sample healthy, simple, fresh meals.
Gas chromatography was performed on fat samples and provided relative amounts of fatty acids expressed on a fat basis in g/100 g fat.
Fat samples were collected from the neck and stored at -20°C until used for androstenone measurements.
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