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However, this number will have been limited by the number of genes surveyed, the low signal-to-noise ratio of certain genes (e.g. Per2), and the use of experimental samples likely to include a large proportion of the surrounding hypothalamic tissue.
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For all arrays RNA was assayed using direct labeling of experimental samples (Cy 3) and Stratagene Universal Human Reference RNA (Cy 5).
The signals obtained from reference RNA have been used for normalisation of experimental samples.
Second, we have shown that RNA-Seq can be used to assess microarray probe quality, and that this can be done using a relatively small number of experimental samples.
Considering the slight deviation of the obtained parameter n from 1, the interpretation of the impedance of experimental samples using capacitance element gives satisfactory results.
The platform consists of experimental samples using Affymetrix GeneChip Grapevine Genome Array.
It would have been a familiar sight in labs around the world, where researchers routinely use tape to test the adhesive properties of experimental samples.
In order to minimize the use of experimental animals, these blood samples were obtained from fish sampled for intestines (see below).
These potential population/breed specific CNVRs can be considered as good candidates for determining breed-specific characteristics, although it is necessary to confirm phenotypic effects of these CNVs using more experimental samples.
Ethical approval for collection of patient stool samples and use of experimental animals for the experimental procedures was obtained from the Scientific and Ethical Review Office, EPHI (006/2003).
The number of animals used in the experimental samples was the minimal possible to attain statistical significance.
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