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To confirm the use of equal amounts of RNA in each experiment, all samples were checked in parallel for β-actin mRNA expression.
PCRs for the housekeeping gene GAPDH served as a positive control for the RT reactions and the use of equal amounts of cell line-specific template RNA (Figure 2B).
Of note, non-target GAPDH message could also be amplified, albeit less efficiently and to the same extent in both IP groups; this finding has been described before [23], and verified the use of equal amounts of input material.
To confirm the use of equal amounts of RNA in each experiment all samples were checked in parallel for β-actin mRNA expression.
An additional phosphorylation band detected at 42 kDa suggests autophosphorylation of ZmCK2α and showed the use of equal amounts of the kinase in the assays.
The first round of sequencing involved the use of equal amounts of all five libraries (EF, F, E, MeJA, and C) and ligating them to the 454 adapters as described in the original 454 paper [ 102].
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GAPDH mRNA expression was analysed in parallel to confirm the use of equal amount of cDNAs in each reaction and all quantitations were normalised to this endogenous control.
To control the recovery of histones we used immunoblotting of equal amounts of cell samples and of acid-extracted proteins, as calculated from the volumes of the starting material and of the final protein extract.
Twelve protein fractions isolated from those cells were equalized by protein content and used for phosphorylation of equal amounts of the purified recombinant GroES in the presence of [γ-P]ATP.
The two-colour microarray experiment was conducted as a common reference design using a reference consisting of equal amounts of total-RNA from all samples.
The reference material, which was used for all studies, consisted of equal amounts of RNA from both female and male tissues (liver, brain and gonad).
More suggestions(15)
use of excessive amounts
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use of stoichiometric amounts
use of significant amounts
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use of appropriate amounts
use of equal levels
use of vast amounts
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use of equal questionnaires
use of minimum amounts
use of equal probabilities
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use of equal weights
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