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These differences may partly be explained by the use of different tissue fixation protocols, primary antibody clones and detection systems or a combination of these factors.
Although our protocol for assignment into MLLs was robust to the use of different tissue types, different tissue type pairs varied in their average pairwise genetic differences.
Also, the use of different tissue reconstruction filters affects the CT numbers of coronary arterial plaques [25].
These results indicated the importance of use of different tissue types in gene correlation analysis.
Results from test samples showed that our genotyping protocol was robust to the use of different tissue types.
We could again confirm the importance of the use of different tissue types (e.g. shoot/root under stress and stamen development).
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This differences between the expression of AtWRKY28 [ 116] and BnWRKY28 (this study) in response to ET treatment may be the result of subtle differences in experiments including the use of different tissues (seedlings versus leaves), and/or the ethylene-generating reagents (ACC versus ET) used.
The use of different tissues was tested in a range of studies, including comparisons of data collected from fresh leaves and twigs from the same plant, and dried leaves and stems from the same plant.
First, microarrays are a powerful tool but can be prone to type I errors stemming from the large number of comparisons involved and variation in experimental conditions (e.g., use of different tissues, treatments, ecological types, and species).
The advantage of using autologous cells in transplantation studies prompted us to test our protocol on nine hiPSC lines generated in our laboratories using retroviral transduction of cells of different tissue origin.
We then analyzed the cell specificity of LJK-11 using human tumor cell lines of different tissue origins, including the lung adenocarcinoma cell line A549, cervical cancer cell line Hela, gastrointestinal cancer cell line HGC-27, and breast cancer cell line MDA-MB-453.
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