Another reason could be the use of different tagging material at the two hospitals (100 ml Gastrografin at hospital A and 20 ml Iomeron at hospital B).
The PCR products were at equimolar concentrations (26.1 ng each) with two exceptions; amplification products from the buffalo were added at double concentration (52.2 ng), and PCR products generated from the zebra template used twice the number of different tags.
Each tag method has its advantages and disadvantages, and combination of different tags and developing of new tags had been proposed and performed.
The identification of these interactions in the PCA dataset [4] but not in the HT-Y2H or LC datasets [38] [39] may be due to differences between the Y2H and PCA methodologies [83] or to differences resulting from the use of different fusion tags in Y2H and PCA screening strategies.
In addition, the use of different fusion tags mean that alternative purification protocols are required for each protein expressed from a different vector, increasing the time it takes to find successful expression and purification conditions.
To analyze how effectively the markers of different tag sets have been put to use, we determined the counts of tagSNPs used to describe each population and tagSNPs that could tag SNPs from multiple populations.
The blocking technique performed by SRB and PRB enables the use of different slots by arriving tags and staying tags, which prevents the former from colliding with the latter.
Liu and co-workers presented a novel strategy for simultaneous detection of multiple DNA targets based on the use of different encoding metal ions as tags.
Much effort has been spent to develop methods to address these issues ([17] [21]; and references therein), including the use of different strains, selective growth conditions, fusion tags, co-expression with binding partners, and expression of independently folded structural domains.
They used 96 different tags and carried out 96 different purifications (i.e. as many tags and purifications as samples).
