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Furthermore, the lack standardization of ADC values (mainly due to the use of different sequences and different gradient-shape design by the manufacturers) still suggests caution in the routine application of the technique.
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The deposited data sets may contain genomes and gene annotations that differ in their degree of precision and resolution owing to the use of different sequencing methods and annotations.
All five studies (see Table 1) compared cost data for the use of different sequencing platforms.
This is despite the use of different sequencing and genotyping technologies for the construction of the maps.
The use of different sequencing platforms in this study was instrumental in confirming significant and consistent changes in transcriptional activity.
Assembly metrics from these shotgun assemblies is difficult to compare directly due to the use of different sequencing platforms, different PE and MP libraries, and different sequencing coverage.
One possible explanation for these different 16S rRNA sequencing results may be the use of different sequencing approaches, such as using cloned DNA versus genomic DNA as template.
One of the strengths of FunnyBase is the use of different sequence databases (SwissProt [ 40], NCBI UniGene [ 36], and NCBI non-redundant NR [ 41]) to provide separate annotations.
The use of different sequencing platforms, the optimization of library preparation, and data analysis will further improve the reconstruction of viral quasispecies.
Such robustness facilitates the use of different sequencing platforms in actual surveillance and investigations to achieve repeatable results in subtype differentiation.
Although the analysis of somatic mutation data from multiple projects provides interesting insights about the genetic cause of tumor, we need to remember that data can have biases due to the use of different sequencing protocols and variant-calling procedures.
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CEO of Professional Science Editing for Scientists @ prosciediting.com