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The discrepancy could be caused by differences in experimental approach, including the use of different mouse strains and different inoculation routes of tumor cells than those we employed in our present study.
The use of different mouse strains revealed that HVR5 substitution also led to dramatically less adenovirus liver transduction and associated toxicity, whereas HVR5-modified Ad were still able to transduce different cell lines efficiently, including primary hepatocytes.
A second explanation lies in the use of different mouse strains.
It is possible that the use of different mouse genetic backgrounds is the cause of these contrasting results.
The use of different doses in mice for histology and biochemistry, and the use of different mouse Aβ phenotypes for the 2 experiments, do not assist interpretation.
These contrasting reports on Sox9 regulation of lung epithelial lung branching may be due to the use of different mouse genetic backgrounds by Perl and Chang.
Similar(51)
While we consider the results of both studies very similar, the differences concerning median lifespan and survival could be due to the various differences in design: use of different mice strains, use of different β1-blockers, and different doses and ways of administration.
Using a combination of different mouse alleles, we can greatly facilitate the understanding of which candidate gene at a particular disease locus is associated with the disease in humans, and also provide functional analysis of variants through an allelic series, including analysis of hypomorph and hypermorph point mutations, and knockout and overexpression alleles.
The divergent results in this respect between the latter and our study may originate from several factors, such as the use of a different mouse strain, the application of different dosages, the region studied (colon vs. ileum,) and the type of inflammation (only delayed type hypersensitivity reaction after administration of a second dose vs. acute inflammation in our study).
To avoid introducing confounding parameters due to use of a different mouse strain or a different age/developmental stage in the NOD strain, the mRNA level for each gene in the diseased lacrimal glands of the 20-week old male mice was compared to the level of expression of the same gene in the healthy lacrimal glands from female NOD mice of the same age.
To compare the normalized data from dysplasia, normal lung tissue from transgenic mouse, tumor cells and non-transgenic of different mice, we used the Significance Analysis of Microarrays (SAM) algorithm (ArrayTrack), which contains a sliding scale for false discovery rate (FDR) of significantly up- and down-regulated genes [69].
More suggestions(15)
use of transgenic mouse
use of comparable mouse
use of irradiated mouse
use of different network
use of different cellphone
use of mutant mouse
use of monoclonal mouse
use of different cell
use of different summer
use of inbred mouse
use of frozen mouse
use of different peptide
use of commercial mouse
use of different vocal
use of different collision
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