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Other possible causes of the observed conflicting results between studies include species differences and use of different experimental models.
The reasons for such disparate results are currently unknown, but may relate to the use of different experimental systems and/or to putative differences in self-antigen processing by lymph node stromal cells and dendritic cells.
The use of different experimental conditions and strains prevent us to make meaningful comparisons to earlier expression data (Guida et al. 2011), but we could confirm that 98% of the genes expressed in our strains and conditions are also expressed in any of the replicas in the other study.
Other factors contributing to the difference in test performance among the studies are related to the use of different experimental protocols and criteria of scoring.
The use of different experimental paradigms or estrogen treatment regimens may contribute to these disparate findings.
In experimental science, the term means something else, namely that a result is invariant with respect to the use of different experimental methods.
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CMAs (Critical Method Attributes) such as sensitivity, selectivity and variability are evaluated as a function of different experimental variables using DoEs (design of experiments).
This highlights the robustness of the effect which has now been demonstrated using a number of different experimental paradigms [1] [3].
We confirmed this finding using a number of different experimental approaches, showing that the effect relies on p-Akt2-selective phosphorylation of mitochondrial nNOS, after kinase translocation to the organelles.
Functional information is largely enriched using an overlay of different experimental results.
This study confirms the expected role of TBP and SNAP50 in binding to the SL RNA promoter, as observed in several different kinetoplastids using a variety of different experimental techniques [ 14, 24, 35, 46].
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