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Beside methodological differences (use of different cell lines, mode of YopM delivery, method of purification) there is considerable variability between different YopM proteins from different species and serogroups.
One possible explanation for this difference was the use of different cell lines.
The differences can be assigned mainly to the use of different cell lines and cultivation conditions like media composition, aeration mode, pH control and culture type e.g. suspension or immobilized, batch or fed-batch.
In general, we can summarize most of the recent developments to reach clinical application into 2 major trends: the use of different cell sources or the application of biomaterials as a cell-free approach.
Although the reasons for these differences have not been completely explained, the distinct effects of various BPs (e.g., pamidronate, zoledronate, and alendronate) and the use of different cell lines (e.g., human vs. rat and primary vs. cancer) could play a role [25].
The inconsistencies may result from the use of different cell lines or different culture conditions used.
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However, these differences may be due in part to the use of different cells lines or differences in cell culture conditions where, for example, investigators have used 1% FBS [39], 5% FBS [35], [36], [37], [38], [39] or 10% FBS [21].
Thus, there is a possibility that use of different cells yields different results.
Moreover, large-scale computational studies may be hampered by artifacts produced during EST library preparation, e.g. uncertainty concerning the origin of the samples or use of pools of different cell types.
Cell viability was measured by the neutral red assay, an indicator of cytotoxicity used in cultures of different cell lines [23] with the same sensitivity as the MTT assay [24], [25].
Expression of the E1 proteins was analyzed by Western Blot using protein extracts of different cell pools.
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