The omega-3 FAD-type genes in G. hirsutum (AD1 allotetraploid), G. herbaceum (A1 diploid), and G. raimondii (D5 diploid) were cloned and sequenced using a combination of database mining, degenerate primer-based PCR screening, genome resequencing, and gene-specific PCR-based cloning, as described in the Methods.
Therefore, to better understand the functional role of the CatSper channel complex, we set out to determine the evolutionary origins and patterns of CatSpers and CatSperβ using extensive database mining and rigorous phylogenetic analyses, and to analyze the evolutionary rates and functional divergence of CatSpers and CatSperβ.
Using genome database mining and other informatics approaches, we identified and characterized the repertoire of 54 intact "V2R-like" olfactory C family GPCRs in the zebrafish.
In addition, the expression pattern of gene members was studied using database mining and in situ hybridization tools.
The application of these principles to the development of the predictive QSAR models of anticonvulsant agents followed by the use of these models for database mining is shown to afford the discovery of novel anticonvulsant agents.
We generated a list of known and putative Drosophila RBPs using a combination of literature and database mining and expert curation.
To do this, we first generated a list of known and predicted Drosophila RBPs using a combination of literature and database mining and expert curation, and then generated the reagent library (see Materials and Methods).
Recent approaches to integrating the data relevant to AD have involved the use of text mining, bioinformatic databases and network analysis for gene co-expression and protein-protein interactions [ 24- 33].
This not only avoids the need for additional database scans, but also enables the use of existing PSP mining algorithms to mine for NSP.
The frequency is dependent on the presence or not of redundancy but also related to the parameters used for SSRs screening in the database mining.
