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Other approaches include the use of correction algorithms a posteriori to the assembly process.
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Many factors interfere with more accurate approximation of starting dates for evolution and preclude use of elaborate correction algorithms [28] (discussed in the supplement).
This was a favourable situation for the use of batch correction algorithms, and accordingly, the batch effect was perfectly removed by the ComBat algorithm.
Although lower estimates were obtained after the use of the correction algorithms, the proportions relative to the different categories remained approximately the same.
Fig. 11 Test of correction algorithm.
Additional file 5 Test of correction algorithm, right.
Additional file 4 Test of correction algorithm, left.
Additionally, photobleaching in the focal plane may be somewhat nonuniform due to the varying illumination power, which may complicate the use of any photobleaching correction algorithms.
The three different cases that were evaluated were no correction, use of a basic correction algorithm and use of the advanced algorithm as described in this paper.
To facilitate general use, the correction algorithm is presented as an Excel spreadsheet (Supporting Information (SI)).
A better application would use a drift correction algorithm to calculate the errors of the 32.768KHz oscillator that drives the realtime clock.
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