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Choice of GP also motivated the use of coronal section for clearest possible definition.
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Immunohistochemical stainings were performed on the same animals using parallel sets of coronal sections.
This method uses a set of coronal sections that cover the entire posterior cortex of the rat.
The frozen tissue block was cut into 20 series of coronal sections, using a cryostat.
The tracings of coronal sections were then used to generate dorsal view reconstructions of the furthest distal points where labeled axons were found, as well as axon density plots of projections arising from injection sites.
Medulla oblongata was sectioned using a freezing microtome, and a series of coronal sections (30 µm) was collected and stored in anti-freeze at −20°C for subsequent histological processing.
Seven series of coronal sections were cut throughout the brain.
A series of coronal sections of 50 μm were cut through the striatum with a vibratome and processed for immunohistochemistry using the immunoperoxidase method.
Use of coronal, sagittal, and coronal oblique sections and several different MRI sequences is important to increase the ability to detect lesions of these structures.
The ratio of empty lacunae (empty lacunae/the total number of osteocytes) was calculated for each femoral head using a coronal section taken at the maximal femoral width.
To quantify donor cells, we used every tenth coronal section (20 µm) throughout the entire spinal cord (a mean of 25 sections per animal).
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