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Our objective was to assess the extent to which access to genome-scale enrichment patterns would provide strain-engineering insights not reasonably accessible through the use of conventional sequencing.
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Our conclusions highlight the importance of sediment supply and local tectonism, and caution against a priori use of conventional sequence stratigraphic and fill-and-spill models to decipher the stratigraphic evolution of actively-deforming mud-dominated continental margins.
Length refers to the length of conventional sequence data used in error calculations.
Nonetheless, a marked variation in sensitivity and negative predictive value has been reported in the literature, reflecting the intrinsic drawbacks of current diagnostic criteria, which are based mainly on the use of conventional CMR pulse sequences.
Such validation studies have previously proved valuable in exploring both the power and the limitations of phylogenies to reliably reconstruct known chains of transmission using conventional sequencing techniques (for example, [ 6, 7, 13, 14]).
The method is designed to sacrifice little flexibility — it works on previously acquired data and allows the use of conventional high-SNR pulse sequences with no extra scan time.
Designed primer pairs, labeled at the 5′ end using one of the conventional sequencing dyes 6-FAM or HEX (Applied Biosystems, Carlsbad, California, USA), were further accessed among 43 morphologically divergent cultivars of L. indica and five Lagerstroemia species with PCR amplification.
The overall dimensions of the ITER Tokamak and the particular assembly sequence preclude the use of conventional optical metrology such as mechanical jigs and traditional dimensional control equipment used for the assembly of smaller, previous generation, fusion devices.
To estimate the accuracy of the GS 20 sequence, custom primers were designed to check all possible frame shift errors encountered in the preliminary DOGMA annotation of the GS 20 sequence of both genomes using PCR and conventional sequencing.
To assess the accuracy of the GS 20 sequence, over 45 kilobases of sequence were generated for each genome using conventional sequencing.
Global down regulation of miRNAs in mouse T cells using PCR and conventional sequencing approach was reported before [ 91].
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