To evaluate the ultrasound images, two types of computerized image texture analyses programmed in Interactive Data Language (IDL) were used (Interactive Data Language, Research Systems, Inc., Boulder, Colorado, USA).
In scientific experiments, subjective evaluation has been replaced by the use of objective computerized image analysers.
The use of computerized or digital image analysis (DIA) has greatly facilitated the evaluation of ST.
The number of Foxp3-positive cells and tumor-infiltrating lymphocytes (TILs) was counted using a computerized image analysis system composed of a DP70 CCD (charge-coupled device) camera (Olympus, Tokyo, Japan) mounted on an Olympus AX70 light microscope (Olympus).
In each slide, DNA-damage extent of 50 randomly chosen cells was analyzed and assessed quantitatively by visual scoring or using the fluorescence microscope with an excitation filter of 546 nm and a barrier filter of 590 nm using computerized image analysis system (Komet Version 3.1. Kinetic Imaging, Liverpool, UK).
Areas of osteolytic lesions were measured using the computerized image analysis system MorphoExpert (Exploranova).
The area of the region of interest was measured and, after background correction, the number of immunopositive nuclei was quantified using a computerized image analysis system (Leica Qwin version 2.3, Leica Microsystems Imaging Solutions).
Images of tissue sections were recorded using a computerized image analysis system (AxioVision, Carl Zeiss).
The area of osteolytic lesions was measured using a computerized image analysis system (IMPAX; AGFA, Cologne, Germany) and results were expressed in square millimeters.
