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MRAC is the appropriate body in PNG to grant approval for the later use of clinical samples in research, as was the case for this study.
Among them, 40 were excluded from analysis because they were diagnosed as having secondary HLH, or their parents did not provide permission for use of clinical samples.
As the KSHV lentiviral library consists of 31 KSHV ORFs we decided initially to perform our immunogenic screen with moDCs transduced with up to three different KSHV ORFs simultaneously, in order to make the experiments more manageable and to make the best use of clinical samples.
All patients included in this study provided written informed consent for the use of clinical samples.
Use of clinical samples in this study was approved by the Ethics Committee of the University of Hong Kong.
Ethical approval for use of clinical samples has been obtained from the local research ethics committee, reference 09/H0606/5.
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Using spoligotyping of clinical samples without culture confirmation, we were able to diagnose an M. bovis infection and rapidly identify 2 cases of recurrent tuberculosis in patients with the same spoligotyping pattern in both followup specimens and the initial M. tuberculosis strain isolated several months earlier.
A rapid diagnostic assay for SPPV using different type of clinical samples would be useful for outbreak management.
The applicability of the new assay in routine diagnostic testing was evaluated using a set of clinical samples.
The limit of detection of the assay was determined by using serial dilutions of clinical samples and was 0.1– 0.01 parasites/μL of blood, and NASBA was found to be quantitative.
SELDI offers the advantages of rapid, high throughput screening using small volumes of clinical samples, and includes rapidity and reproducibility in the screening of protein expression profiles (also known as 'phenomic fingerprints').
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