Sentence examples for use of callus from inspiring English sources
For particle bombardment, similar to the Agrobacterium-mediated transformation, the use of embryogenic suspension calli (D0E) was better as compared to the use of callus stage D0C and embryoid stages of D1, D2, and D3 (Table 2).
One disadvantage to the use of embryogenic callus- and seed-derived callus systems is that they generally have limited lifespans (months) of usefulness before they cease to be regenerable.
Consequently, we recommend avoiding direct use of embryogenic callus for transformation experiments and instead completing a number of preliminary proliferation cycles to increase the quantity of PEMs.
The maintenance conditions for the embryogenic callus cultures, particularly a high auxin to cytokinin ratio, the age of the culture (optimum for 7-10 mofths of proliferation) and the use of a yellow callus phenotype, were the most important factors for achieving highly efficient transformation (> 90%).
Petri-dishes (9 cm dia), each containing 25 ml of medium were used for callus induction experiment.
The results suggested that the use of MS can enhance callus mineralization and biomechanical strength in the callus region.
Particle bombardment of callus using these two promoters exhibited expression patterns comparable to the maize Ubi1 promoter and much higher than that using the 35S promoter [ 60].
Radiographic assessment was used to confirm the presence of callus at the fracture site (Fig. 4) and incorporation of the strut allograft (Fig. 5).
A comparison of the maximum fresh weight of callus obtained using 2,4-D (15.83 μM) and 2,4-D (11.31 μM) with BAP (0.44 μM) showed decline in callus fresh weight 145 mg and 154 mg respectively.
Microprojectile bombardment of callus was performed using the procedure of Somers et al. [ 20].
The obtained radiographs were used for objective evaluation of the bone healing using criteria such as bone union, size of callus formation, degree of bone comminution, pin angulation, and radiograph callus index.
