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It is known that an inverse correlation exists between the size of a gene's family and its use of alternatively spliced isoforms in humans, mice and worms.
The use of alternatively spliced RCLs appears to be a wide spread strategy in insects to diversify the range of target proteinases that can be controlled by a single serpin gene [ 56- 58].
These results are summarized in Table 2. Splicing was also examined in human R2B transcripts where the use of alternatively spliced exons was virtually identical to that observed in the mouse genes.
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The use of two alternatively tagged proteins in combination with corresponding anti-tag antibodies, followed by secondary antibodies labeled with different size gold particles (10 nm and 5 nm gold) allows the determination of the relative distribution of two different membrane proteins [12].
We used two sets of alternatively and constitutively spliced exons curated from independent sources as controls (see Methods).
The use of kappa distributions, alternatively, results in a more correct value of the main parameter of the theory of hot plasma splitting.
While exon arrays [ 10- 12] and RNA-sequencing [ 13, 14] are being used for expression profiling of alternatively spliced forms, it is possible to identify the expression status of some transcripts using gene expression data, which already exists for a huge variety of tissues and conditions, across different species.
The cadherins are all clustered on chromosome 5 and consist of a tandem array of alternatively used 5' exons and common 3' exons.
Mouse is the primary model organism used in experimental studies of alternatively spliced isoforms.
The power of exon-based expression analyses lies in the identification of alternatively used transcripts in tumours, which can provide new insights into the aberrant circuits of the most aggressive tumour fraction, harbouring a plethora of options for therapeutic interventions.
Alternatively, use of paddy straw for composting is the best alternative to manage this resource, along with its use for restoration of soil health (Gaind et al. 2008).
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