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To address this issue, a proper initialization of the gains and the use of additional optimization constraints will be considered.
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A number of additional optimizations are performed.
While some of those markers might have yielded better gel-based data (i.e., stronger target bands) upon PCR optimization, qPCR data were produced without the use of additional time and resources necessary for optimization.
It combines the structural modifications stage that gives the maximum heat recovery, with the cost optimization stage which optimizes the use of additional exchanger area.
The hormone concentrations and timing of application for this study were motivated by the existing literature; however, the use of a scaffold-free system may justify additional optimization of hormone dosing, timing, and duration.
Additional optimization of the frequency of injections and the cell numbers would be necessary for practical application.
The parameters we used should serve as a starting point for subsequent investigations and additional optimization of conditions may be required for unique tissues not assessed in our study.
No additional optimization of the protein structures was carried out.
The comparison shows that additional optimization of the kinetic parameters is required.
In such a case, limits can be set for additional optimization of the quantitation (Figure 3A).
Therefore, for these metabolites, additional optimization of the extraction procedure will be required.
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CEO of Professional Science Editing for Scientists @ prosciediting.com