Sentence examples for use of a touchdown from inspiring English sources

Amplification may have been assisted by the use of a touchdown PCR program and the use of the QIAGEN Multiplex PCR Master Mix, which enhances the likelihood of successful PCR amplification from primers with differing annealing temperatures.

Another strength of our assay is the use of a touchdown PCR covering a range of annealing temperatures (between 62°C and 54°C) to ensure that all the primer pairs hybridise specifically to the template DNA and adequate amounts of PCR product are finally obtained.

All PCR reaction were performed using Hi fidelity Taq-Gold (Amersham, Buckinghamshire, UK) to minimize polymerase error in a volume of 25 µl using a touchdown method, starting with a 60°C annealing temperature and ending at 50°C or 48°C.

PCR reactions contained 20 ng of template, 0.33 µ m of each primer, 200 µ m each nucleotide, 50 m m KCl, 0.1% Tween-20, 1.5 m m MgCl2, 35 m m Tris base and 15 m m Tris HCl in a final volume of 30 µl, using a touchdown protocol with annealing temperature beginning at 55°C and stepping down to 50°C.

The exons and the 5′ region of intron 1 of KIT were amplified using a touchdown procedure and sequenced as described previously (Table S5) (Ishida et al. 2006).

A new and potentially less complex and lighter way to reduce the shock loads at touchdown is the use of a crushable shield underneath the lander platform.

For ospA and ospC, the first round of amplification was carried out using a touchdown protocol; after an initial denaturation step of 95°C for 5 min, 2 cycles of 95°C for 1 min, 64°C for 1 min, 72°C for 1 min were run, followed by decreasing the annealing temperature by 1°C per 2 cycles until reaching an annealing temperature of 55°C, used for the next 17 cycles.

Cycling conditions were: 5 min initial denaturation at 95 °C, followed by 40 cycles of 10 s at 95 °C, 30 s at 65 °C, 30 s at 65 °C, but during the first 10 cycles, we used a touchdown mode of 1 °C per cycle, 10 s at 72 °C, and a heteroduplex formation consisting of 10 s at 95 °C and 20 s at 40 °C.

The CEN gene fragment was amplified using a touchdown PCR program consisting of 95°C for 15 minutes, 10 cycles of 95°C for 30 seconds, 65°C for 30 seconds decreasing 1°C per cycle, 72°C for 2 minutes, followed by 25 cycles of 95°C for 30 seconds, 55°C for 30 seconds to 72°C for 2 minutes and a final extension of 72°C for 7 minutes.

All PCR reactions was performed in an Agilent Sure Cycler 8800 using a touchdown amplification profile consisting of an initial denaturation at 95 °C for 5 min followed by 40 cycles of denaturation at 95 °C for 2 min, annealing at 65 °C for 90 s, extension at 72 °C for 2 min with a final extension at 72 °C for 10 min.

Briefly, 24 STR markers in regions showing loss of heterozygosity in cancer were amplified using a touchdown PCR protocol.