Sentence examples for use of a third primer from inspiring English sources

One primer from each pair was modified on the 5′ end with an engineered sequence (CAG tag 5′-CAGTCGGGCGTCATCA-3′) to enable use of a third primer in the PCR that was fluorescently labeled with one of three dyes (6-FAM, NED, or VIC; Applied Biosystems, Culver City, California, USA).

One primer from each pair was extended on the 5′-end with an engineered sequence (M13R tag 5′-GGAAACAGCTATGACCAT-3′) to enable the use of a third primer identical to the M13R (Schuelke 2000), and a GTTT 'pigtail' was added to the 5′-end of the untagged primer to facilitate accurate genotyping (Brownstein et al. 1996).

One primer from each pair was modified on the 5′ end with an engineered sequence (CAG tag 5′-CAGTCGGGCGTCATCA-3′) to enable use of a third primer in the PCR (identical to the CAG tag) that was fluorescently labeled for detection.

We added a CAG sequence to the 5′ end of one of each of the primer pairs to facilitate use of a third, fluorescently labeled primer in the PCR.

The forward primer from each locus was 5′ modified with an engineered "CAG-tag" sequence (5′-CAGTCGGGCGTCATCA-3′) to enable use of a third, fluorescently labeled primer (identical to the CAG-tag) in PCR.

Therefore, the use of a second set of primers (with different sequences than those used for the initial PCR and sequencing run) is recommended for the re-sequencing of questionable cases.

To amplify the 5' half of the stem, we used one primer upstream of the cassette and a second primer that comprises the loop sequence at its 3' end and sequences downstream of the cassette at its 5' end (thus "jumping over" the 3' half of the stem).

For nested-PCR, 1 μL PCR product of the first round of PCR was used as template for the second PCR, making use of a nested primer and the common 3' or 5' primer.

The first PCR strategy involved the use of a standard two primer PCR while the second strategy used a single specific primer in a step-up PCR protocol.

For this reason, use of the third forward primer, OF640, is recommended for the detection, amplification and quantification of difficult target napA genes from ϵ-proteobacteria.

Deviations from the manufacturer's instructions included use of a modified oligo dT) primer to create first-strand cDNA, and a modified priming strategy during second-strand cDNA synthesis to promote the amplification of larger mRNA molecules.