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Future research warrants the use of a stringent study design with a control group to determine program efficacy.
It is possible that our use of a stringent case definition for diarrhea may have missed an association with more mild gastrointestinal symptoms such as nausea.
The use of a stringent definition of a positive result was implemented in this study in order to maintain high specificity [13].
The use of a stringent Bonferroni adjustment to correct for multiple testing highlighted the large number of metabolites that decreased immediately post surgery and then did not change between T3 and T6 (FIGURE 1).
Despite the use of a stringent threshold (P ≤ 0·001) for detecting QTLs in the two populations, about one-third are common (Table 3).
First, the combined use of a stringent hot start PCR and touch-down cycling conditions reduces the formation of non-specific amplification products and primer-dimers.
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The use of such a stringent gene selection method that uses both multiple testing correction and a fold-change cutoff did not significantly affect our conclusions.
The North American study group applied an appropriate, genome-wide significance level, and use of such a stringent threshold has immediately led to replication in our independent panel with a level of significance that makes the association indisputable.
This is true because the use of a less stringent test for protection of characters in the graphic medium casts doubt on the vitality of the more stringent story being told test for graphic characters.
In the case of safety biomarkers, we wanted to retain the null hypotheses, therefore the use of a more stringent Bonferroni p-value adjustment threshold (p-value <0.0023) for testing 22 independent immune markers would likely be non-conservative, resulting in false negatives.
This reduction is again due to use of a more stringent cut-off used for whole genome de novo searches combined with use of updated genome assemblies.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com