Sentence examples for use of a mutant from inspiring English sources

This can be done by recapitulating the morphant phenotype with a second, non-overlapping Morpholino targeting the same mRNA or by confirmation of the observed phenotypes by use of a mutant strain or dominant-negative methods.

Our analysis is therefore complementary to the previous elegant demonstration in which the first AGO1-dependent layer of defense was exposed through the use of a mutant TCV that did not produce the P38 suppressor of AGO1 [17].

One elegant solution for genotyping pollen makes use of a mutant allele that is molecularly tagged with a cell autonomous marker gene, such as β-glucuronidase (GUS) [12] or any fluorescent protein that is expressed in pollen [15] [17].

We observed that cells in which strong activation of IRE1α was partially blocked by use of a mutant form of IRE1α were more resistant than control cells to the glucotoxic effects of chronic high glucose on insulin gene expression.

Heat inactivation of GGT (HI GGT) or the use of a mutant lacking catalytic activity (GGTm) demonstrated that only the active catalytic form of GGT can induce miR-155 expression.

2) Requiring the use of a mutant transposon and hence existing transposition vectors must be mutated.

However, a potential limitation of the use of a deletion mutant library for persister mutant screens is that compensatory mutations could mask the persister defective phenotype, which may lead to an inability to identify critical persister genes.

Ptf1a morpholinos alone do not completely remove all ACs (Jusuf et al., 2011), so we made use of a tilling mutant from the Zebrafish Mutation Project (ptf1a sa126 ).

These strategies include the construction of a synthetic streptavidin gene, the installation of a transcription terminator, and the use of a sporulation mutant strain.

For this purpose it was determined if the use of a p53 mutant, which has all its six acetylated lysine residues in its oligomerization C-terminal domain mutated, was still able to induce VRK1 downregulation.

Use of a K48R mutant ubiquitin showed deficient chain elongation.