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They fear arrest since the use of a kit by necessity, involves possession of a controlled substance.
Albumin was measured by use of a kit (QuantiChrom™ BCG Albumin Assay Kit BioAssay Systemss, Hayward, CA, USA) according to the manufacturer's instructions.
The relative gene expression of each sample, normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), was calculated by the formula: Annexin staining was conducted with the use of a kit (Annexin V-FITC Apoptosis detection kit; BD Pharmingen, San Jose, CA, USA).
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DNA extraction was performed on 200 μl of homogenized milk through use of a commercial kit (Qiagen DNeasy kit, Valencia, CA) following the manufacturer's instructions.
Genomic DNA was extracted from microbiome samples by use of a commercial kit (Biostic Bacteremia kit; Mo Bio Laboratories, Carlsbad, CA).
The ST2146 isotype was determined by the use of a commercial kit (Mouse-hybridoma subtyping kit, Boehringer Mannheim, Monza, Italy).
Serum concentrations of IL-18 were measured by enzyme-linked immunosorbent assay (ELISA) with the use of a commercial kit (IL-18 ELISA Kit; MBL, Nagoya, Japan).
Oxidized LDL (oxLDL) was determined by use of a commercial kit (Mercodia, Uppsala, Sweden) and antibodies against phosphorylcholine (anti-PC) by use of a commercial kit (Athera CVDefine™, Stockholm, Sweden) as described by the manufacturers.
This included the use of a trauma kit, CPR and a defibrillator.
Hence, the use of a suitable kit for the cDNA synthesis is of great importance.
Protease digestion was performed by use of a Recoverall kit™ (Applied Biosystems, AM1975).
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