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Both IFA tests and fluorescence staining require the use of a fluorescence microscope, limiting their usefulness in low resource settings.
A further improvement in the efficiency of the surface modification experiments resulted from the use of a fluorescence read out of the fibrinogen adsorption.
Immediately, the slides were examined with use of a fluorescence microscope.
For fluorescence detection, slides were examined by use of a fluorescence microscope (Zeiss Axiovert 135, Carl Zeiss, Heidenheim, Germany).
With the use of a fluorescence automated microscopy screen, small molecules that decrease cholesterol in the LSOs of NPC1 mutant cultured cells were identified [39].
In vivo visualization of GFP expression in salivary glands or in vitro cultured spheres was accomplished by the use of a fluorescence microscope (Olympus IMT-2, Japan) or Confocal Laser Scanning Microscopy (CSLM) (Leica TCS SP2), with or without PKH-26 cell linker visualization (Sigma-Aldrich, St . Louis MO, P-9691).
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Changes in cell cycle kinetics in tumor cells treated with NPRL2 with or without cisplatin were analyzed by flow cytometry (with use of a fluorescence-activated cell sorter [FACS]) using an APO-BRDU KIT (BD Biosciences Pharmingen).
The use of fluorescence-based techniques needs to be supplemented by the use of a fluorescence-quenching step (for example, Sudan Black).
The poration within the PEG microspheres was examined through the use of a confocal fluorescence microscope by taking slices of the collected fluorescence through the depth of the sphere.
In the present study, the use of a sensitive fluorescence imaging system to monitor thousands of cells afforded us the ability to discriminate subtle Ca2+ changes.
At timed intervals, non-adherent cells were removed and adherent cells were quantified with the use of a SAFIRE fluorescence plate reader.
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