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We thank Y. Gotoh, S. Hattori, M. Matsuda, A. Miyawaki, J. Miyazaki, L. Stephen, and T. Tsuboi for expression plasmids; A. Takada for an antibody; H. Sawa for use of a confocal microscope; A. Nanbo for useful comments; N. Toyoda for technical assistance; S. Darmanin for critical reading of the manuscript; and members of our laboratory for helpful discussions.
The use of a confocal laser scan microscopy based technique enabled us to identify cell type and test cell viability in tissue slices maintained for at least 4 days in culture.
In the undercooled melt of Pd40Cu30Ni10P20 alloy, the solidification behavior including the nucleation and growth of crystals at the micrometer level has been observed in situ by use of a confocal scanning laser microscope combined with an infrared image furnace.
The immunofluorescently labeled leptospires were examined with the use of a confocal LSM 510 META immunofluorescence microscope (Zeiss, Germany).
I thank Dr. Prabhu Ramamoorthy for participating in preliminary experiments, Ms. Qian Wang for help with confocal microscopy, Dr. Charles Nichols and the Department of Pharmacology LSUHSCC) for the use of a confocal microscope and Dr. June Liu for critically reading the manuscript.
However, the measurement and quantification procedures that, for example, involve the use of a confocal microscopy have severely limited throughput rates.
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The confocal images were acquired by use of a laser confocal scanning microscope (TCS SP5/AOBS/Tandem, Leica, Germany) at Korea Basic Science Institute, Gwangju Center.
The lens was designed for a high magnification (37X) to enable use of a larger confocal pinhole (50 μm), while maintaining a compact tube length (85 mm).
The use of a large confocal aperture provided a higher relative contrast for the motion of erythrocytes at all locations tested.
The old tool is the use of a high-resolution confocal microscope that can provide resolution for up to 0.5 micrometers -- less than 1/200,000 of an inch.
The use of a laser scanning confocal microscope gives the advantages of far-field microscopy (possible sample perturbation as in the case of near-field technique is prevented) with spatial resolution well below the diffraction limit, and fast laser scanning for fast data acquisition and lower sample photodegradation.
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