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More recently, a striking demonstration of its potential has been to use it to reconstitute the expression of the multisubunit T-cell receptor CD3 complex in bone marrow-derived stem cells transduced with retroviral vectors carrying up to four CD3 genes separated by 2A sequences [ 10, 11].
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To confirm that the expressed Prnp is functional, we used it to stably reconstitute Prnp-silenced PK1 cells (PK1 Prnp-kd) and challenged a heterogeneous pool of these cells with mouse RML prions.
"How are you going to use that to reconstitute the whole bird?" By far the greatest potential losses of genetic diversity are in developing nations, where imported livestock often supplants indigenous breeds and damages the environment.
Mr. Wolf said the new sanctions regime "is designed to make clear that the international community is interposing no barriers on goods going to Iraq's civilian economy, but that it is determined to keep rigorous controls on things that Iraq could use to reconstitute its military capability".
If the first phase of the information age celebrated an assortment of virtual realities, the next phase may be all about using technology to reconstitute the body that it never quite succeeded in allowing us to transcend.
Concerned Muslim intellectuals must engage with this critique to understand it, but not use it as the primary motivation to reconstitute our understanding of Islam or reformulate Muslim institutions.
A fourth diplotype, cE211/cK211, was observed when recombinant cDNA from the Alabama BSE case was used to reconstitute its diplotype.
Bone marrow transplantation using hematopoietic stem cells is a common procedure used to reconstitute the bone marrow and regenerate the immune system after full or partial myeloablative therapy to eradicate blood based cancers.
Additionally, changes in anisotropy are not observed when a 181 bp fluorophore-labeled DNA, comparable in length to the DNA used to reconstitute the F18N18F nucleosomes, was used as the substrate in the reaction.
One possibility for these differences is in the sequence used to reconstitute the nucleosomes; indeed, it is known that different positioning sequences give rise to different dynamic nucleosome states.
Although InChI keys cannot be used to reconstitute chemical structure without lookup tables, their use has enabled cross-database chemical searches using common web search engines.
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