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Based on these results, we designed specific primers and a probe for use in the quantitative PCR assay.
High discriminating power, high sensitivity towards branching amalgamated with negligible degeneracy offer proposed descriptors a vast potential for use in the quantitative structure activity/property/toxicity relationships so as to facilitate drug design.
Sampling frame for service providers was based on patient-reported service use in the quantitative study, patient interviews and advice of previously injured lay research advisers.
Total cellular RNA for use in the quantitative RT-PCR assay was isolated from confluent cultured cells and tumor tissues by using an RNeasy Mini Kit (Qiagen, Valencia, CA) and then treated with RNase-free DNase I (Ambion, Austin, TX).
Previously, we have reported the design and development of an internally controlled multiplex real-time PCR NAD assay for both P. aeruginosa and Burkholderia species for use in the quantitative detection and identification of these GNB-NE in biocontaminated HPW under laboratory conditions [ 27].
The benefits of obtaining the emission data for stationary and mobile sources are 2-fold: the general qualitative characterization of the segments, and the use in the quantitative estimation of the exposure in each district using dispersion modeling and exposure characterization modeling.
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Thallous chromate, formula Tl2CrO4, is best used in the quantitative analysis of thallium, after any thallic ion, Tl3+, present in the sample has been reduced to the thallous state, Tl+.
Patient exit interviews (survey) were used in the quantitative methods.
This essay is a crash course in the metrics used in the quantitative analysis of startups by early-stage investors.
The primers used in the quantitative PCR are listed in the Table S1.
The primers used in the quantitative PCR are shown in Additional file 1: Table S1.
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