Sentence examples for use in the assay from inspiring English sources

In order to avoid interaction with outside environment after collection, the larvae were maintained in the bee combs prior to their use in the assay.

The density of the cell cultures was adjusted photometrically so that cultures contained approx. 5×108 cfu (colony forming unit)/ml prior to their use in the assay.

Where indicated, toxin was preincubated with pre-immune or immune sera for 30 min at 4°C prior to use in the assay.

The use in the assay of overlapping 15-mer peptides covering all the PfCS102 sequence should have overcome the problem of genetic restriction and possibly increased the level of responses.

To this end, human umbilical vein endothelial cells (HUVECs) were first cultured on fibronectin-coated 6-well plates in endothelial basal medium-2 (EBM-2) (PromoCell, USA) and then treated with 0, 20 or 100 IU/ml EPO for 48 hours before their use in the assay.

Notwithstanding that determination of soil-sorbed prion infectivity by SCA imposes stringent requirements, the specific use in the assay of (also) an arable sandy loam enriched of all its (heat-stable) natural components, and tissue homogenates, aimed at approximating field conditions.

The NPB 5.69 M stock was diluted in 50 mM bis-tris/5% glycerol (pH 7.0) for use in the assays.

The P-CoA 20 mM stock was diluted in 50 mM bis-tris (pH 7.0) for use in the assays.

These coatings were conditioned in deionized water for 14 days prior to their use in the assays described here.

These were dissolved in water at 10% weight by volume for use in the assays.

The following day the cells were washed and counted for use in the assays.