Sentence examples for use in subsequent rounds from inspiring English sources

Based on the sequence of this amplicon, additional target-specific PCR primers were designed for use in subsequent rounds of 3′- and 5′-extension using DNA walking technique to eventually obtain a C. bombicola genomic sequence containing an open-reading-frame putatively identified as a glucosyltransferase (gtf-1).

DNA that is eluted in this fashion is PCR-amplified to seed a new, enriched library for use in subsequent rounds of selection.

For the more complex scenario of informative test choice (SIM-2), we assume that in each round two tests can be performed, but only one result can published, i.e. used in subsequent rounds.

Throughout the analyses all splice variants of all identified homologues accepted for further analysis were used in subsequent rounds of RBH (eleven candidate homologues were found to possess splice variants, see Table S1).

The resulting colonies were tested for resistance to indolotryptolines, and resistant colonies were used in subsequent rounds of backcrossing.

Then, 100 μL out of 2 mL phage solution were directly used in subsequent rounds of biopanning as described above.

Seed stocks made from crystals of the mutant enzymes were used in subsequent rounds of crystallization trials.

DNA from these sorted beads was recovered by PCR to generate an enriched library and used in subsequent rounds of selection.

Based on the contribution score, only relevant features are used in subsequent round(s) of classification.

The most active mutants were selected, characterised and used as a template in subsequent rounds of ep-PCR.

A variety of algorithms in multi-objective evolutionary optimisation (e.g. ref. 111 116) use members of the Pareto front as the choice of which 'parents' to use for mutation and recombination in subsequent rounds.