SREHP/MBP fusion protein, which was among the first recombinant proteins used in serodiagnosis of ALA was reported to have a sensitivity and specificity of 74% and 55%, respectively.
Several studies reported the use of PPD in serodiagnosis of tuberculosis infection with high sensitivity [ 11, 12].
The results highlight the immunosensitive and immunospecific nature of Rv3425, which shows promise for use in the serodiagnosis of TB.
The role of PPD in serodiagnosis of TB, with sensitivity as high as 92%, was reported in Warao and Creole populations [ 10].
43 We believe that L. infantum-rHsp83 is a good antigen for use in the serodiagnosis of leishmaniases.
Therefore, this study aimed to determine whether a newly developed rOMPs indirect ELISA (rOMPs I-ELISA), based on combination of rOMP25, rOMP28 and rOMP31of B. melitensis, has a potential benefit for use in the serodiagnosis of brucellosis.
Subsequently, direct-ELISA and dot-blot immunoassays were designed, using crude preparation SAG1 AP conjugate, to explore its value in serodiagnosis of human toxoplasmosis.
Another report showed 92% sensitivity and 96% specificity in serodiagnosis of canine sarcoptic mange by ELISA [ 41].
Despite the satisfactory results of serological tests, development of reliable and standard reagents remains a major constraint in serodiagnosis of T. gondii infection.
