Sentence examples for use in evolutionary engineering from inspiring English sources
These results show that our system is stable, effective, and suitable for use in evolutionary engineering applications.
Stabilization is not the only objective for which the approach is useful, and others could include the selection of residues for nondisruptive destabilizing mutations to reduce the lifetime of proteins, or to generate proteins that can be used to screen for second-site revertants in evolutionary engineering strategies.
See the review by Cakar and co-workers for recent approaches in evolutionary engineering of strains [ 42].
The use of evolutionary engineering has proven to be instrumental in obtaining phenotypes of microorganisms with improved properties [ 58- 60].
A long-term adaptation in repetitive batch cultures in shake flasks using a cocktail of 12 different inhibitors and a long-term chemostat adaptation using spruce hydrolysate were used as evolutionary engineering strategies to improve the inhibitor tolerance in the metabolically engineered xylose utilizing Saccharomyces cerevisiae strain, TMB3400.
We have used an evolutionary engineering approach that depends on a quadruple hexokinase deletion xylose-fermenting S. cerevisiae strain to select for growth on D-xylose in the presence of high D-glucose concentrations.
Using evolutionary engineering strategies in batch and chemostat cultivations we have generated three evolved strains that show significantly better tolerance to inhibitors in spruce hydrolysate and displayed a shorter time for overall fermentation of sugars compared to the parental strain.
Improvements in qXylose of up to one magnitude order were achieved using evolutionary engineering, and strains capable of co-utilization of glucose and xylose, and of cellobiose and xylose were obtained [ 25- 28].
The strain showing the highest μmax (strain IBB10A02), determined as the increase in optical density at 600 nm (OD600) over time, was used for further evolutionary engineering by repetitive batches.
The concentration of salts, trace elements and vitamins were exactly the same as used in the medium for evolutionary engineering in shake flasks.
The recombinant xylose utilizing S. cerevisiae TMB3400 [ 26] was used as a parental strain in the evolutionary engineering strategies.
