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It is of no use in a real-time situation.
Tests with simulated demand suggest that the procedures are well suited for use in a real-time traveller information system.
All primer pairs (see Table 2) were optimised at a 60 °C annealing temperature using traditional PCR prior to use in a real-time PCR system.
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This method greatly reduces the computational expense and can be used in a real-time fashion.
These tasks are used in a real-time agent architecture called ARTIS.
In the future, these data could be used in a real-time surveillance system by medical causes of death.
The tools are being used in a real-time systems course as a basis for student projects.
The experimental results show that the frequency tracking method works quite well and fast enough to be used in a real-time controller.
The dual-labeled TaqMan probes (ProOligo, France) for these primers were specifically designed and used in a real-time PCR assay.
TaqMan-minor groove binder (MGB) probes were used in a real-time PCR-based assay for the rapid and accurate detection of hepatitis B virus (HBV YMDDD mutants.
Therefore, CTC detection and characterization may be valuable tools for refining prognosis, and CTCs can be used in a real-time tumor biopsy for designing individually tailored therapy against CRC.
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