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Additionally, current, nonbiological, chemical synthesis methods for CdS NPs are typically energy intensive and use high concentrations of hazardous cadmium precursors.
Recently, novel in-office bleaching products that use high concentrations of hydrogen peroxide (HP) have made in-office treatments easier.
Due to its daily use, high concentrations of BPA are observed in wastewater and in wastewater sludge (0.004 1.36 mg kg−1).
Additionally, because of highly inefficient endosomal escape for delivery into the cytosol,[ 31] most delivery methods use high concentrations of components, and this can give rise to cellular toxicity.
Since our cells are grown under high levels of glucose (DMEM, containing 25 mmol/l glucose), it is necessary to use high concentrations of glucosamine in order to appreciate its effect in the presence of high glucose.
We opted to use high concentrations of FFAs because in the past, we have demonstrated that FFAs at these concentrations blocked insulin- and mixed meal induced muscle microvascular recruitment in healthy humans (6, 7).
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One major obstacle is that to keep the structural integrity of DNA nanostructures, we need to use high concentration of cations such as magnesium.
It is recommended not to use high concentration of TMAC for Chattanooga and Pride Mountain shale even in the presence of other additives.
These methods require large amounts of the target molecule, as they use high concentration of the molecule(s) to be crystallized.
For example when poly-His-tags are used for purification purposes it is common to use high concentration of imidazol in protein elution steps.
Limitations in availability of Aotus sera did not allow us to use higher concentrations of serum antibodies.
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