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Five growth chambers (Model: ZSX1500GS manufactured by The Shanghai Jing Wins and Scientific Equipment Co., Ltd., China) were adjusted for dry runs, one week prior to their actual use for the experiments.
In order to allow the adaptation to the new oxygen concentrations, hNSC and IhNSC were cultured for 4 5 passages after the shift before use for the experiments.
The sample was then freeze-dried and the dried extract was stored at 4°C before use for the experiments.
A 15-mM solution was freshly prepared with tetrahydroflan (THF) (Sigma-Aldrich, St . Louis MO, USA) immediately before use for the experiments.
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Java is used for the experiments.
A SB320-50DW was used for the experiments.
Table 2 Value of the parameters used for the experiments.
The supernatant was collected and used for the experiments.
Deionized (DI) water was used for the experiments.
Only early passages (<50) were used for the experiments.
The cells were used for the experiments until passage 4 (P4).
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