Following the reinsertion of the stem, the remaining doughy cement is used for punctual fixation onto the femoral resection surface.
Punctal occlusion after topical timolol determines a decrement of beta-blocker plasma levels, so the use of punctual occlusion after every drop application is advised [ 62].
Then in our patient cohort, we conducted a simultaneous search for punctual mutations and gene rearrangements using qPCR-HRM in the prospective group and qPCR alone in the retrospective group (as these patients had already been assessed for qualitative alterations), then SQF-PCR in both groups as a confirmatory quantitative approach (Figure 1).
This holds for punctual sources and free-space propagation.
* Significant δ ≤ 1 and β > 0, evidence for punctual evolution.
** Significant β, node density artefact present; δ < 1 and significant β, evidence for punctual evolution.
These trends indicate that evidence for punctual evolution is strongest for the climatic niches and somewhat less for the habitat and trophic niches.
The element crack closure is taken into account using the punctual restriction criterion.
The spatial variability of total Hg in soil using punctual concentrations and grouping of sites according to Cf values are given in Figure 3.
DOI: http://dx.doi.org/10.7554/eLife.08150.017 As an example, we have expanded our analysis using Equations (1- 3) to also include an analysis of the experimental data using the model with punctual removal, i.e. we determined best-fit parameters for this punctual model and present graphs of its agreement to both data sets over time (Author response image 1).
Only qualitative alterations were found with a sensitivity similar to that obtained using classical molecular techniques for screening punctual mutations.
