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It is important to note that AAVs exist in the muscle cells as extrachromosomal elements and do not integrate, which increases the safety of their use for long term expression of antibodies or other proteins (Greig et al., 2016).
The best test to use for long term follow-up remains to be investigated.
Formal cardiac rehabilitation programmes are designed to introduce patients at an early stage after their cardiac event to health behaviours such as exercise, dietary modification and relaxation which they can continue to use for long term health maintenance [ 2].
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This new tracer technology is a stable, non-invasive, non-toxic tracer that can be used for long term fluid identification.
However, its advantages become apparent in a persistent environment where the file system is used for long term storage purposes.
Fatigue lifetime estimation of load bearing parts of construction is a key factor in a design of technique used for long term operation.
The phosphoglycerate kinase (PGK) and the EF1α promoters have also been effectively used for long term constitutive transgene expression in ESCs.
Lentiviral vectors have been used for long term systemic expression of genes in mice[23], and we therefore tested whether hIL-7 could be delivered to immunodeficient mice using such an approach.
The MDS is currently used for long term care placement assessment by the Social Welfare Department.
Freezing at −80 °C or in liquid nitrogen (−196 °C) [11,13] is most often used for long term storage; for short term storage −20 to −28 °C is preferred [10,14].
21 This threshold is now used for long term symptoms, 22 and a more recent study proposed 8.7 m/s as a threshold for acute soft tissue injury.
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