Richard Attenborough used "darling" to pin people down and his phrase "Entirely up to you, darling" was a masterstroke of passive-aggressive coercion.
It is a charge he has answered many times, insisting he only uses "darling" because he can't remember peoples' names, "so it's nothing to do with being a ham", and that, "I don't break out into tears anything like as often as everybody says I do".
Furthermore, the analysis of bout-length distribution using Darling's test revealed that behaviours walking (p > 0.01) and scanning-while-walking (p > 0.001) deviated from exponentiality due to the presence of mixtures of exponentials.
Next, we used Mauve (Darling et al. 2004, 2010) to infer sets of putatively orthologous genes among the 27 E. coli and Shigella strains.
Synteny of the recovered fosmid inserts with regions of existing genomes and intergenic region similarities was determined using "Mauve" (Darling et al. 2010).
We used progressiveMauve (Darling et al. 2010) to produce whole-genome alignments of M. smegmatis for input into ClonalFrame (Didelot and Falush 2007).
Once assembly correction was complete, we compared the genomic arrangement of each strain with that of the complete wMel genome using Mauve (Darling et al. 2004).
Next, we used Mauve (Darling et al. 2004) to align these rough plastome assemblies to one or more published reference sequences (listed above).
Location of sampling, genome data, and accession numbers are listed in table 1. Genomes were aligned using ProgressiveMauve (Darling et al. 2010, version 2.3.1) with iterative refinement and allowing rearrangements.
We then used Mauve (Darling et al. 2004) to align the wMel and wMelCS genomes to the wMelPop assembly, and exported the core genome regions with minimum LCB length of 100.
Whole-genome alignments were done using Mauve (Darling et al. 2004) with one of the copies of the large duplication in CRt masked, and the resulting permutations file was used as input for MGR (Multiple Genome Rearrangements) (Bourque and Pevzner 2002), with the circular genome setting, to infer the ancestral gene order and phylogenetic tree.
