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We were constrained by a lack of alternative instruments validated in the Arabic language or Saudi Arabian population that we might use as standards.
Different compounds from anthocyanidins, flavan-3-ols, proanthocyanidins, flavanones, flavones, and flavonols sub-classes were first optimized for use as standards for liquid chromatography mass spectrometry (LC MS) with UV detection.
TNF-α, TLR-4, and GADPH clones for use as standards were prepared by PCR from cDNA derived from PM and cloned into p-Drive vector (Qiagen, S.p.A., Milano, Italy).
For each qPCR experiment, an oligonucleotide was synthesized that encoded the entire amplicon or the amplicon minus the sequence between the 3' ends of the two primers for use as standards and positive controls.
We extracted 32 "disease-gene families" to use as standards for evaluating GLAD4U performance before and after thresholding.
RNA transcripts corresponding to the VP6 of NCDV strain were generated for use as standards in the analysis of sensitivity of the assay.
Similar(52)
Ascorbic acid (AA) and BHT were used as standards.
N-acetyl chitooligosaccharides, glucose, and cellobiose were used as standards.
VH and PVH, used as standards, were synthesised earlier [37].
Spectroscopically characterized synthetic impurities were used as standards.
Glucose and cellobiose were used as standards in (c).
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