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This is a fascinating pioneer, but it could use another round of seasoning.
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GS linker and 6XHis-tag PCR fragment was added with the using another round of overlap PCR.
For both 3'-RACE and 5'-RACE, following PCR amplification we used another round of nested PCR.
The largest teachers' union, NASUWT, said the tables were being used for "another round of denigration of schools and teachers by the government".
But the opposition accused Morrison of a "cynical and dishonest" campaign to use the NDIS as cover for "another round of harsh cuts to vulnerable Australians", saying the previous increase to the Medicare levy was only ever intended to part-fund the landmark scheme.
Using the LL3 sub-clone, 5 overlapping fragments were generated and used for another round of complementation tests.
In detail, for every SIRL i, i > 0, the partially decoded frame X ^ i - 1, created at the previous level, is used in another round of SI generation by means of OBMEC[34], where the SAD criterion is used as the error metric during block matching since the hash frame is no longer involved in the motion estimation.
The colonies were either used in a phage ELISA (see below) to identify antigen binders or they were pooled and used in another round of panning.
This is true since the "fresh" test data used in another round of double cross-validation are not truly "fresh" but a subsample of the entire data set (same for the training data).
Parameters were then estimated on the resulting tree and used for another round of heuristic search.
Probe sets showing upregulation were then used for another round of 10SOM clustering analyses (4 × 5), but this time as individual time points.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com