Add three frames and use the tile command to view two of the three frames.
Measure the size of the tiles you want to use and any tile spacers or grout.
While it doesn't get bitterly cold where you live, some people do have freezing conditions, and the tile they use needs to be made to withstand freeze/thaw cycles without crumbling.
For each sample, 54 sections (6 × 9) were captured and subsequently stitched using the tile scan feature of the Leica LAS AF 2.1.1 software to allow complete visualization of the striatum.
For long projects, you may need to stop using the tile saw and refill the reservoir before continuing.
Whole-brain images were taken using the tile scan feature and a plan-apochromat 25× (0.95 NA) lens at the following settings: 0.55-μm z-dimension steps, 1024 × 1024 pixels, 10% tile overlap, and 12-bit data acquisition.
The mah-jongg set also includes a pair of dice, a quantity of tokens or chips used for scorekeeping, and a rack used to keep the tiles upright and to keep their faces hidden from other players.
Practice using the tile cutter with some scrap tile or less expensive pieces.
All the sleeping room floors were covered with tiles (tile size 19.5 cm × 19.5 cm), and we used the tiles on the floor as markers, so that we could always distribute the containers in the same pattern.
Note that the tiles within the same row and column should use the same tile width and height, respectively.
