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PMN were re-suspended in 1 mL PBS (4°C), stored on ice until use, and counted using a hemocytometer (VWR, Mississauga, ON, Canada).
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These orders gave election officials greater flexibility to use and count provisional ballots.
After 48 h, cells were detached using trypsin and counted using a haematocytometer (VWR International, Darmstadt, Germany).
After 48 h, cells were detached using trypsin and counted using a haematocytometer counting chamber (VWR International, Darmstadt, Germany).
An unbiased counting frame was used, and counting was performed using an × 20 lens at a total magnification of × 794.
We harvested cells using trypsin and counted them using Vi-CELL software (Beckman Coulter, Margency, France).
Thereafter, the cells were collected using trypsin and counted in a Bürker-Türk.
Cells were serially passaged using trypsin/EDTA (Invitrogen) and counted using a Z2 Coulter Particle Counter.
Bacteria were cultured on blood agar at 37°C for 24 hours using serial dilutions and counted using a routine plate count method.
The microchaetae from the whole field were overlaid using Adobe Photoshop 9.1 and counted using Image J (Version 1.42n).
Reads were aligned using TopHat2 [ 42] and counted using HTseq [ 43] in Python.
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