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After centrifugation at 500 g for 10 min at 4°C, supernatants were collected and stored at −40°C until use, and cells were resuspended in 1 mL of MEM.
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For experiments involving macrophages, the cell line J774.1 was used, and cells were grown and stimulated in complete DMEM (same recipe as RPMI – Sigma-Aldrich).
The project is based on earlier research in which we engineered miniature kidneys using biomaterials and cells.
He has been taught to wear baseball caps, backwards, and to use beepers and cell phones.
We had incorrectly assumed that people would just use laptops and cell phones.
Use online and cell phone alerts as a reminder.
Briefly, cells were dissociated using Accutase and cell numbers determined.
All FACS studies were performed using single cell suspensions, and cells were stained using standard protocols.
The thieves switched warehouses at least three times and used and discarded cell phones regularly, officials said.
Open image in new window Fig. 3 Cell fusion model using cells expressing mtDsRed and cells expressing mtGFP.
We currently can use cells, and we can use scaffolds, but we can't yet use them together in the USA.
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