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Note that we did not use an internal control of very abundant RNA because it often affects the ability of non-abundant RNA to prime.
As the major components of the NM undergo appreciable changes during PCa development, it was not possible to use an internal control to ensure equal loading on the gels.
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RNU6B was used an internal control.
We normalized the values using an internal control, RpL3.
Cyclophilin primers were used an internal control (Ambion, Austin, TX).
For this reason, we developed a standard operating procedure using an internal control.
Intensities among different arrays were normalized using an internal control as suggested by manufacturer.
In some experiments, 18S primers were used an internal control (Qiagen, Valencia, CA).
Ubiquitin was used as an internal control.
OsUBQ5 was used as an internal control.
OsActin was used as an internal control.
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